A draft version of the EPPO standard on the organisation of interlaboratory comparison studies (EPPO 2014b) was used for guidance during the TPS setup. TPS results and additionally generated data were used to evaluate the following performance criteria: analytical sensitivity, analytical specificity, diagnostic sensitivity, diagnostic specificity, repeatability and robustness following EPPO standard PM7/98 (EPPO 2014a). warted potato tissue in round 1, and resting spore suspensions in round 2).
Two TPS rounds were organised focussing on different test matrices (i.e. We aim to fill this gap by generating validation data for three molecular tests, including both DNA extraction and amplification, in an international test performance study (TPS). endobioticum standard PM7/28 (EPPO 2004) lies with pathotype identification using different bioassays, and no molecular tests for pathogen detection or identification are included. The main focus of the current version of the European and Mediterranean Plant Protection Organization (EPPO) S. 2006, Przetakiewicz 2015b) and phytosanitary measures heavily rely on pathotype identification. To date, 39 pathotypes of the fungus have been described (Baayen et al.
These resting spores, which can remain viable and infectious in soil for decades (Laidlaw 1985 Przetakiewicz 2015a), together with the lack of successful chemical control agents (Hampson 1993), present a major challenge to potato production. Robust resting spores are formed in the warted tissue and are released into the surrounding soil when host tissue decays. endobioticum induces tumor-like growth (galls or warts) in host tissues of susceptible potato cultivars resulting in yield losses up to 100% (Hampson 1993). is the causal agent of potato wart disease and is considered one of the most important quarantine organisms of cultivated potatoes (Smith et al.
The cosmopolitan soil-borne obligate biotrophic fungus Synchytrium endobioticum (Schilb.) Perc.
#Nic smada update
Tests regarded fit for purpose for routine testing of wart material and resting spore suspensions are proposed for the update of EPPO standard PM7/28(1) Synchytrium endobioticum. For overall accuracy and analytical sensitivity, the van Gent-Pelzer significantly outperforms the van den Boogert and Bonants tests and is regarded as the test of choice when identifying S. endobioticum in resting spore suspensions, the van den Boogert and van Gent-Pelzer tests significantly outperform the Bonants test for diagnostic sensitivity and diagnostic specificity. When using the tests for detection and identification of S. endobioticum in warted potato tissue, no significant differences were observed for diagnostic sensitivity, diagnostic specificity, overall accuracy, analytical sensitivity and robustness. round 1: warted potato tissue, and round 2: resting spore suspensions. Two TPS rounds were organised focussing on different test matrices, i.e. endobioticum, and the pathotype 1(D1) identification test described by Bonants et al. ( European Journal of Plant Pathology, 126, 129-133, 2010) for the detection of S. ( European Journal of Plant Pathology 113, 47–57, 2005), and van Gent-Pelzer et al. Tests regarded fit for purpose for routine testing of wart material and resting spore suspensions are proposed for the update of EPPO standard PM7/28(1) Synchytrium endobioticum.Īn international test performance study (TPS) was organised to generate validation data for three molecular Synchytrium endobioticum tests: van den Boogert et al.
(European Journal of Plant Pathology, 126, 129-133, 2010) for the detection of S. (European Journal of Plant Pathology 113, 47-57, 2005), and van Gent-Pelzer et al. An international test performance study (TPS) was organised to generate validation data for three molecular Synchytrium endobioticum tests: van den Boogert et al.
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